Category: Colorimetry lab report

This data table is the measurements of each concentration and absorbance of each of the six solutions. The measurements were found using a colorimeter. The linear graph shown is the six solutions plotted based on their molarity and absorbance. The graph shows the higher the Molarity, the higher the absorbance. The purpose of this lab is to determine the concentration of a colorful solution.

Four solutions and a colorimeter were used to determine the concentration of a CuSO 4 solution with unknown concentration.

The four solutions were then graphed, and the slope of the graph and Beer's Law was used to determine the concentration of the unknown solution. The concentration of the unknown solution is. Sources of error could of been from not pouring the correct amount of CuSO 4 and distilled water for each test tube or the colorimeter could have been off.

General Chemistry Monika Borkowski site map. Conclusion: The purpose of this lab is to determine the concentration of a colorful solution. Show Comments 0 and Tags. In order to comment on this portfolio you must be logged in to the school or organization it is associated with. If you have a Digication account, you may log in below: username: password:.Styrofoam Cup Calorimetry Experiment Introduction Calorimetry is a process of measuring the amount of heat involved in a chemical reaction or other process.

In this experiment, I used thermometer to see if the chemicals is gaining or losing heat energy. Materials Stopwatch 2 Thermometers 2 Styrofoam cups 0. Procedure 1. Prepare the materials and use lab coat and gloves for protection 2. Draw tables to record the data of the initial temperature and the temperature for every 30 seconds until four minutes of each chemicals and the combined chemicals. Prepare 2 Styrofoam cups. Cut a little bit of one Styrofoam cup and place it on the top of the other cup, so it will looks like a shaker.

Make sure you can still open it so you can pour the chemicals later. Make a hole on the top of the cup to put the thermometer. Take ml of a Sodium Hydroxide and Hydrochloric Acid with concentration of 0.

Put it in two separated beakers and measure the temperature of both mixtures. Write the data. Quickly and carefully close the Styrofoam cup with the other Styrofoam and put the thermometer in. Count the initial temperature. Stir the combined chemicals slowly with the thermometer in case you dont have a stirrer and record the temperature changes for seconds.

After seconds, pour the combined chemicals out of the Styrofoam. Repeat step for 1M and 2M, but make sure youve washed the Styrofoam before repeating the steps.

Lab 5 - Determination of an Equilibrium Constant

Hypothesis The heat will increase at first, but it will decrease after some times. This process can be categorized as Exothermic reaction, because this releases energy during the process. Temperature of combined chemicals during reaction Time second 0 30 60 90 0. Styrofoam Cup Calorimetry Experiment Questions and answers 1. Why is it important that these reactions be run in a styrofoam cup as opposed to a beaker?

Styrofoam cup is a very good insulator. The loss of energy to the experiments surrounding will be less in the styrofoam cup compare to the beaker.Colorimetry colourimetry is also referred to as colorimetric analysis colourimetric analysis.

colorimetry lab report

Colorimetry colourimetry is an analytical technique used by Chemists to determine the concentration of a coloured solution. A solution is coloured because it absorbs different amounts of the different wavelengths that make up "white light".

A colorimeter colourimeter is an instrument used to measure the absorbance of light by a coloured solution. The wavelength chosen for colorimetric analysis is that which gives maximum absorption for the coloured solution. We can use a "colour wheel" to determine the colour of light to use. The intensity of the colour of a solution is related to the concentration of the solution. The more concentrated the solution is, the more intense the colour will be. To determine the concentration of a solution using colorimetry: 1 Determine the wavelength colour of light to use for the colorimetric analysis.

This is the calibration curve. Please do not block ads on this website. Many solutions can be coloured. You may already be familiar with some coloured aqueous solutions, for example:. The colour we see is the colour of the light that is transmitted through the solution. If we shine white light through an aqueous solution of copper sulfate, the solution appears to be blue because it is transmitting the blue wavelengths of white light and absorbing other wavelengths such as red so that very little of these other wavelengths are transmitted through the solution to your eye.

The colour of the solution is due to the wavelengths of light that are not absorbed as "white light" passes through the solution. Now, if we dilute the original copper sulfate solution by adding more water, the blue colour becomes less intense, it is a lighter blue colour because the solution is not absorbing as much of the other wavelengths as before:.

If we were given an aqueous solution of copper sulfate of unknown concentration, solution Awe might be able to take a guess at its concentration by looking at its colour:. The "blueness" of solution A seems to lie about half-way between that of the concentrated 0. Guessing the concentration of the solution in this way gives us a kind of "ball park" value for the concentration, but it is unlikely to result in us knowing the concentration of the solution accurately or precisely.

But there might be a better way. We have already noted that there is a relationship between the intensity of the colour of solutions and their concentration.

And we have seen that a concentrated blue solution will absorb more of the "non-blue" wavelengths of light than a more dilute solution, and we have a machine, a colorimeter colourimeterthat can measure the amount of absorbance for us! Play the game now! Colorimetry colourimetry is the name of the method we use to determine the concentration of a substance by measuring the relative absorption of light with respect to known concentrations of the substance.

A colorimeter colourimeter is literally a meter to measure colour. Therefore, we need to choose a wavelength of light to shine through our solution. The solution must be able to absorb measurable amounts of this wavelength. Different concentrations of the solution must absorb different amounts of this wavelength, and this difference must be significant that is, much larger than the error inherent in the measurment. If a solution is blue, like CuSO 4 aqwe would not use a wavelength corresponding to blue light because very little of this is absorbed most is being transmitted.

Similarly, lots of the green and indigo wavelengths are not absorbed but are transmitted. Therefore we would choose to shine light from the "red-orange-yellow" part of the spectrum at this solution because these are the wavelengths that are being absorbed.

We can use a "colour wheel" to decide which is most likely to be the best colour of light to shine on our solution:. If our solution is blue, we choose a light colour that is complementary to blue, that is, we choose the colour that is opposite this colour in the colour wheel, in this case we choose an orange light to shine on our blue solution. Colour of Solution Colour of Light to Use in Colorimeter yellow purple orange blue red green purple yellow blue orange green red.

Now, it may be possible to select an appropriate wavelength for the light our colorimeter uses, but it is quite likely that our colorimeter will use coloured filters in order to "filter out" absorb the wavelengths of light that we do not want from "white light".Calibration curves are one of the most ubiquitous and essential procedures in analytical chemistry.

This experiment used a phosphorus stock solution of known concentration to make a series of calibration standards. The absorbances of these standards were then measured to create a calibration curve.

colorimetry lab report

The absorbances of two solutions of unknown concentration were then measured. These values met with expected values of concentration for the unknown solutions. The importance of determining the concentration of phosphorus in phosphates of many forms—ortho, organic, poly is mainly environmental—phosphate pollution remains a major problem due to its overuse in detergents and large-scale fertilizer; the subsequent runoff of phosphates into the environment can cause significant harm to plants and animals Lim.

The main method utilized for quantitative phosphate analysis is through solution spectrophotometry, which is what is used in this experiment. Also utilized is the practice of producing a calibration curve. Calibration is incredibly essential to practically all aspects in analytical chemistry. Without a sense of calibration, measurements have no meaning in relation to one another. Thus, the concept of the calibration curve is created.

The basic theory behind the calibration curve is that if there exists a series of known values eg concentration and their resulting measurements eg absorbancethe values of the measurements of unknowns can be calculated. For example, this experiment makes use of a series of phosphorus standard solutions with known concentrations and their resulting absorbances measured by a spectrophotometer to create an equation for a line of concentration vs.

This equation is then used to calculate the concentration of an unknown phosphorus solution. The overall grand purpose of the calibration curve is to determine the unknown based on what is known. Spectrophotometry remains a relatively fast, accurate method of calculating the concentration of substances in solution by way of measuring their absorbance at specific wavelengths.


A calibration curve and its subsequent linear equation is used in this experiment. This experiment relies on the usage of a spectrophotometer to measure the concentration of the phosphorus in various solutions after reaction with Reagent C. The intensity of the color [and thus the absorbance] will be in direct proportion with the concentration of phosphorus in this sample Lim.

The spectrophotometer is used to measure all of the absorbances for the solutions. The purpose of this experiment is to gain practice in the creation of calibration curves and their application to use them to calculate unknown values from their measurements, based on known values and their respective measurements. The specific calibration curve created for this experiment is concentration of phosphorus vs.

The application of the calibration curve in this specific instance is to find the concentration of phosphorus in two unknown samples based on their absorbances and the calibration curve. The creation of the calibration curve also gives practice in the technique of creating a series of standards.

The procedure described in this section is from the Agilent Method 1 procedure in the paper that was given to the class by the instructor; all credit for the ideation of the following procedure should be given to Sharon Lim of Agilent Technologies, Inc.Colorimetrymeasurement of the wavelength and the intensity of electromagnetic radiation in the visible region of the spectrum.

colorimetry lab report

It is used extensively for identification and determination of concentrations of substances that absorb light. A simple application of this expression is found in comparing intensities of radiation transmitted through layers of different thicknesses of two solutions of the same absorbing substance, one with a known concentration, the other unknown.

If a photoelectric cell instead of the eye is used to compare intensities, the instrument is called a photoelectric colorimeter. In colorimetry, frequently the entire visible spectrum white light is used, and consequently the complementary colour of the one absorbed is observed as transmitted light.

Lab 5 - Determination of an Equilibrium Constant

If monochromatic light or a narrow band of radiation is used, the instrument is called a spectrophotometer. It is not limited to the visible spectrum and is often employed to make measurements in the ultraviolet and infrared regions. The spectrophotometer has largely replaced the colorimeter.

Most of the chemical elements and a large number of compounds may be determined colorimetrically or spectrophotometrically, frequently at concentrations smaller than one part of the constituent in several hundred million parts of solution.

Info Print Cite. Submit Feedback. Thank you for your feedback. Colorimetry chemistry. See Article History. Get exclusive access to content from our First Edition with your subscription. Subscribe today. Learn More in these related Britannica articles:. A variety of instruments are used in this field. The most sophisticated, the spectrophotometers, analyze light in terms of the amount of energy present at each spectral wavelength. The emittance curves for light sources see figure are typical spectrophotometer results, as is the reflectance….

Formulated by German mathematician and chemist August Beer init states that the absorptive capacity of a dissolved substance is directly proportional to its concentration in…. History at your fingertips. Sign up here to see what happened On This Dayevery day in your inbox!

Email address. By signing up, you agree to our Privacy Notice. Be on the lookout for your Britannica newsletter to get trusted stories delivered right to your inbox. More About. David Madore - Colors and Colorimetry.In the deep past, when science was predominantly a matter for philosophy paper topicspeople spoke of different energies and imagined heat as a special concept, giving it a separate place among nature energies — thus, heat energy coexisted with other substances of a system and, say, electrical or vital energy.

From a point of view of contemporary science, there exists only one energy stored in a body — the internal energy, which has a multitude of forms and reveals its appearance as kinetic, electrical or heat energy.

Therefore, the form of energy such as heat is conceived only as coupled with energy changes and is always associated with a heat flow. One may say in a calorimetry lab reportthat calorimeters are the instruments employed for the measurement of the amount of energy that exchanged within a given period of time in the form of a heat flow.

Students often seek someone who can write a research paper for them because modern calorimetry has grown as a complex and sophisticated set of methods. For centuries, it has been known as a highly effective method in natural sciences.

Nowadays, an extensive base for progress in classical physical chemistry and thermodynamics exists due to the possibility of precise measurement of heat of reaction, heat capacity, the heat of combustion, the heat of fusion as well as other caloric quantities. With the progress in computer science and microelectronics, the classical approaches in calorimetry and a calorimetry lab report format have been much revised since it became possible to create and develop new fields of application and new types of calorimeters.

Taking into account how many students choose to do their GCSE ICT coursework using newly-designed calorimetry methods, one could say that contemporary calorimetry experiences growing interest from different fields of science, which now are eager to employ this cheap, easy and powerful approach for various investigative purposes.

Many fields, such as pharmacy, medicine, biology and biochemistry as well as material and food science benefit from using modern calorimetry. It enables scientists to determinate the energy content of fuels and conduct safety investigations, also one could easily find a research paper to buy related to the usage of calorimetry for food quality control. More detailed examples of successful applications are illustrated below:. Get help with any kind of assignment - from a high school essay to a PhD dissertation.

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Lab 4 - Calorimetry

Calorimetry lab report format: introduction to the calorimetry methods. How it works Student places an order. Writers make their offers. Price calculator. You'll have day warranty to request free revisions. Our Price Competitors' price is calculated using statistical data on writers' offers on Studybay.

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How is calorimetry used today? Calorimetry lab report format: the applicability and usefulness of calorimetry for natural and non-natural sciences Many fields, such as pharmacy, medicine, biology and biochemistry as well as material and food science benefit from using modern calorimetry.

More detailed examples of successful applications are illustrated below: - a life sciences example. Regardless of the frictional and incidental heat, all living organisms produce heat as the result of metabolic reactions in the cells. The heat production significantly differs, depending on the temperature of the organism and other parameters such as nutrients, respiration, and atmospheric conditions.

Naturally, the heat production remains constant if the conditions of the environment and the organism temperature are kept constant too. Hence, according to a calorimetry lab report format, the same cells produce a characteristic amount of heat under the constant conditions.The method we will use is called "the indophenol blue method" or "phenate method.

Many modifications of this method are available. The one we will use is based on Solorzano Limnol Oceanogr. Other modifications of this method can be found in Methods of Soil Analysis Page et al.

Clesceri et al. The phenate method also has been adapted for use on various automated analyzers, such as those manufactured by Technicon, Alpkem and Lachat. See the manual for the specific machine you are using.

Notes : Use test tubes which can accommodate a 10 ml sample. All glassware should be very clean scrubbed with hot water, rinsed with 0. Be sure water for reagents and standards is ammonia free. Ordinary distilled water may contain ammonium- use freshly deionized water. Ammonia is volatile and is present in the air. Many different sources of ammonia can contaminate the samples. Smoking, ammonia detergents, freshly cut grass, opening a bottle of ammonium hydroxide in the laboratory, etc.

Exercise caution. Very turbid sample may need to filtered or centrifuged prior to analysis. Precipitation of calcium and magnesium hydroxide can interfere with the analysis. This can be prevented by addition of a complexing reagent such as citrate or EDTA. Miller and D.

colorimetry lab report

Keeney, eds. Methods of Soil Analysis. American Society of Agronomy, Inc. Store in dark bottle for not more than 1 month. Prepare fresh daily. Dissolve 4. Bring to 1 L with DI water.

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